ENHANCEMENT OF COLLAGEN SYNTHESIS PROCESS ON DIABETIC WOUND BY MERREMIA MAMMOSA ( LOUR .) EXTRACT FRACTI ON
By: Marchianti, Ancah Caesarina Novi
.
Contributor(s): Prameswari, Mega Citra.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(2).Description: 47-50p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
This research aimed
to evaluate the
effect
of fracti
onation of
Merremia
mammosa
Lour
. (
Mm
(Lour.)
) extract on diabetic wound healing
by
observing
the
collagen
synthesis process
and to search the most potent fraction
.
Method
s:
Wistar rats
were
divided into five
groups (n=5),
i.e.,
K-(negative
control
),
K+(positive
control
),
K1 (ethyl acetate fraction), K
2 (water
fraction), and
K3 (n
-hexane fraction).
The
Mm
(Lour.) was extracted with ethanol 70%
, then fractio
nated
by using
three solvents which have
different polarity. The rats were
adapted in
7 d
, then induced
into diabetic by
streptozotocin dose 4
0 mg
/kg
body weight.
The wound
wa
s made by
Morton
ex
cision method. Treatment
was
given
every two days
and
a skin
biopsy
was
done on day 11. Analysis of collagen density wa
s done by
photomicrograph
of histopathology
preparations
in Masson’s
trichome
stained by using trinocular
microscope w
ith
400x
magnification
in 6 fields of
view
, then processed by imageJ
software
and
analyzed by appropriate statistic tool
.
Result
s:
The results of this research showed that
fracti
onation
of
Mm
(Lour.)
extract
significant
ly enhance
d diabetic
wound healing based on
macroscopic (percentage of wound healing) and
collagen density with p
-valu
e<0.05
when compared with negative control
, especially the water
fraction
(p=0.00
0). The
follow
-up
post hoc
analysis
showed that there
was
no
significant (p
=0.989
) or there was
no meaningful difference in the
group of
water fraction
when compared
to posit
ive control.
Conclusio
n:
Water fraction is the extract fraction of
Mm
(Lour.)
which has the most significant
influence on diabetic wound healing showed by
enhancement of collagen synthesis.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020887 |
Objective:
This research aimed
to evaluate the
effect
of fracti
onation of
Merremia
mammosa
Lour
. (
Mm
(Lour.)
) extract on diabetic wound healing
by
observing
the
collagen
synthesis process
and to search the most potent fraction
.
Method
s:
Wistar rats
were
divided into five
groups (n=5),
i.e.,
K-(negative
control
),
K+(positive
control
),
K1 (ethyl acetate fraction), K
2 (water
fraction), and
K3 (n
-hexane fraction).
The
Mm
(Lour.) was extracted with ethanol 70%
, then fractio
nated
by using
three solvents which have
different polarity. The rats were
adapted in
7 d
, then induced
into diabetic by
streptozotocin dose 4
0 mg
/kg
body weight.
The wound
wa
s made by
Morton
ex
cision method. Treatment
was
given
every two days
and
a skin
biopsy
was
done on day 11. Analysis of collagen density wa
s done by
photomicrograph
of histopathology
preparations
in Masson’s
trichome
stained by using trinocular
microscope w
ith
400x
magnification
in 6 fields of
view
, then processed by imageJ
software
and
analyzed by appropriate statistic tool
.
Result
s:
The results of this research showed that
fracti
onation
of
Mm
(Lour.)
extract
significant
ly enhance
d diabetic
wound healing based on
macroscopic (percentage of wound healing) and
collagen density with p
-valu
e<0.05
when compared with negative control
, especially the water
fraction
(p=0.00
0). The
follow
-up
post hoc
analysis
showed that there
was
no
significant (p
=0.989
) or there was
no meaningful difference in the
group of
water fraction
when compared
to posit
ive control.
Conclusio
n:
Water fraction is the extract fraction of
Mm
(Lour.)
which has the most significant
influence on diabetic wound healing showed by
enhancement of collagen synthesis.
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